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Human Gβ1 and Gγ2 were inserted into a pFastDual plasmid and were prepared by insertion of His 6 -tagged human Gβ 1 fragment (BamHI/HindIII site) under the polh promoter and insertion of Gγ 2 fragment (XhoI/SphI site) under the p10 promoter, respectively (Genewiz)
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