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GTP recruitment reactions contained 2.5 μg of membrane in 20 mM HEPES (pH 7.4), 10 mM NaCl, 5 mM MgCl 2 , 0.004% w/v saponin (47036, MilliporeSigma), 0.1% bacitracin (11702, MilliporeSigma), and 500 pM [ 35 S]guanosine 5′-(gamma-thio)triphosphate (PerkinElmer)
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Size:2 oz (30 Day Supply)
1 These agents act on GLP-1Rs in the periphery to increase insulin and inhibit glucagon secretion and in the brain to slow gastric emptying and suppress appetite, leading to weight loss
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