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For each amplification cycle particular threshold cycle (C t ) value was obtained and the difference between C t values of targeted miRNAs and U6 were used to calculate ΔC t

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repeat fasting glucose, HbA1c, and lipid panel at week 8 and 16 per ADA 2026 guidance.* Restart your previous maintenance dose if you regain ≥5 % of body weight or if fasting glucose climbs above 126 mg/dL

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