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Ratiometric analysis of the emitted light corresponding to fluorescence originating within a defined region of the cytoplasm was performed using Metafluor v.7.5 software (Molecular Devices)
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A normalized collision energy of 35 was used for CID fragmentation, prior to MS2 occurring with Rs = 30,000, AGC target of 5E6, and maximal integration time of 140 ms selected
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