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Front Endocrinol (Lausanne) 2021;12 doi: 10.3389/fendo.2021.706978
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Protein extraction and normalization Approximately 100 mg of cortex tissue from each mouse (n = 3/group) was homogenized in liquid nitrogen and 1 mL of lysis buffer (8 M of urea, 0.1% SDS, and 1 mM of PMSF)