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Introduction Subarachnoid hemorrhage (SAH) is a feared disease with a high rate of mortality and disability ( Matrix metalloproteinase-9 (MMP-9), a member of zinc- and calcium-dependent proteolytic enzymes family,( via its promoter binding site to nuclear factor-kappa B (NF-κB) ( Glucagon-like peptide 1 (GLP-1), a gut hormone released from intestinal L cells, is responsible for promoting insulin secretion after food intake in a glucose-dependent manner ( Therefore, in this study, we hypothesize that Ex-4 is neuroprotective by reducing BBB damage of EBI through downregulation of downstream targets, NF-κB and MMP-9 via phosphorylation of AMPK (p-AMPK) after experimental SAH model in rats